In an era of growing global health challenges and security threats, *The National Biodefense Strategy* offers a comprehensive exploration of the intricate and multifaceted approaches to national preparedness and defense against biological threats. This anthology, without attributing to individual authors, captures a range of perspectives on biodefense policy, exploring the intersecting domains of public health, homeland security, and international cooperation. While diverse in scope, the collection is cohesive in its thorough examination of proactive and reactive strategies, underscoring the necessity of an integrated, systematic approach to biodefense. The anthology is curated by the White House and the U.S. Government, bringing together voices from pivotal agencies and sectors involved in national security and public health. These contributors collectively embody decades of expertise, shaped by historical and contemporary health crises that illuminate the anthology’s themes. Aligning with movements towards global health security and cooperative international frameworks, the collection captures diverse insights and analyses that address current and emerging biological threats, aiming to fortify national and global resilience. *The National Biodefense Strategy* serves as an indispensable resource for readers eager to understand the complexity of biodefense. As a tapestry of expert analyses, it encourages readers to appreciate the multifaceted strategies and perspectives within biodefense, presenting a multitude of interpretations and insights that enrich its educational value. This anthology invites professionals, policymakers, and scholars to engage in a dialogue on the critical nexus of health security, promoting awareness and understanding of the evolving challenges in this field.