This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
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语言 英语 ● 格式 PDF ● ISBN 9781475753486 ● 编辑 James Pawley ● 出版者 Springer US ● 发布时间 2013 ● 下载 3 时 ● 货币 EUR ● ID 4723848 ● 复制保护 Adobe DRM
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