This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of g RNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.The chapter CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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语言 英语 ● 格式 EPUB ● ISBN 9781071616574 ● 编辑 M. Tofazzal Islam & Kutubuddin Ali Molla ● 出版者 Springer US ● 发布时间 2021 ● 下载 3 时 ● 货币 EUR ● ID 8837654 ● 复制保护 Adobe DRM
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