‘…a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years.’
–Pharmaceutical Research
This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
表中的内容
Contributors vii
Preface
R. Ian Freshney and Mary G. Freshney ix
Preface to Second Edition
R. Ian Freshney xi
List of Abbreviations xiii
Chapter 1. Introduction
R. Ian Freshney 1
Chapter 2. Cell Interaction and Epithelial Differentiation
Nicole Maas-Szabowski, Hans-Jurgen Stark, and Norbert E. Fusenig 31
Chapter 3. The Epidermis
E. Kenneth Parkinson and W. Andrew Yeudall 65
Chapter 4. Culture of Human Mammary Epithelial Cells
Martha R. Stampfer, Paul Yaswen, and Joyce Taylor-Papadimitriou 95
Chapter 5. Culture of Human Cervical Epithelial Cells
Margaret A. Stanley 137
Chapter 6. Human Prostatic Epithelial Cells
Donna M. Peehl 171
Chapter 7. Human Oral Epithelium
Roland G. Grafstrom 195
Chapter 8. Normal Human Bronchial Epithelial Cell Culture
John Wise and John F. Lechner 257
Chapter 9. Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells
Leland G. Dobbs and Robert F. Gonzalez 277
Chapter 10. Isolation and Culture of Intestinal Epithelial Cells
Catherine Booth and Julie A. O’Shea 303
Chapter 11. Isolation and Culture of Animal and Human Hepatocytes
Christiane Guguen-Guillouzo 337
Chapter 12. Culture of Human Urothelium
Jennifer Southgate, John R.W. Masters, and Ludwik K.Trejdosiewicz 381
Chapter 13. Other Epithelial Cells
R. Ian Freshney 401
List of Suppliers 437
Index 443
关于作者
R. Ian Freshney gained a BSc (1960) and Ph D (1964) from Glasgow University. He spent 1965 in Madison, Wisconsin, working on cell interaction and differentiation of embryonic mouse liver before returning to Glasgow to join the Beatson Institute for Cancer Research until 1981. At this time he transferred to Medical Oncology at Glasgow University, where he became a senior lecturer, teaching cell culture and tumor cell biology, with research interests in stromal interaction and differentiation in tumors. He is now an honorary senior research fellow at Glasgow University, focusing his attention on teaching, writing and editing in the field of cell culture.
Mary G. Freshney gained considerable tissue culture experience, initially as a technician with Dr. John Paul, and laterally as a scientific assistant working on hematopoietic stem cells at the Beatson Institute, where she had the major responsibility for the management of the cell culture facility.
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