Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
Tabella dei contenuti
Preface.
Tools and Techniques of Kinetic Analysis.
How do Enzymes Work?
Characterization of Enzyme Activity.
Reversible Enzyme Inhibition.
Irreversible Enzyme Inhibition.
p H Dependence of Enzyme-Catalyzed Reactions.
Two-Substrate Reactions.
Multisite and Cooperative Enzymes.
Immobilized Enzymes.
Interfacial Enzymes.
Transient Phases of Enzymatic Reactions.
Characterization of Enzyme Stability.
Mechanism-Based Inhibition (Leslie J. Copp).
Putting Kinetic Principles into Practice (Kirk L. Parkin).
Use of Enzyme Kinetic Data in the Study of Structure-Function
Relationships of Proteins (Takuji Tanaka and Rickey Y. Yada).
Bibliography.
Index.
Circa l’autore
A. G. MARANGONI, Ph D, is Professor and Canada Research Chair in Food and Soft Materials in the Department of Food Science at the University of Guelph, Ontario, Canada.
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