Protein crystallography has become vital to further understanding the structure and function of many complex biological systems. In recent years, structure determination has progressed tremendously however the quality of crystals and data sets can prevent the best results from being obtained. With contributions from world leading researchers whose software are used worldwide, this book provides a coherent approach on how to handle difficult crystallographic data and how to assess its quality. The chapters will cover all key aspects of protein crystallography, from instrumentation and data processing through to model building. This book also addresses challenges that protein crystallographers will face such as dealing with data from microcrystals and multi protein complexes. This book is ideal for both academics and researchers in industry looking for a comprehensive guide to protein crystallography.
สารบัญ
Protein Crystallography: Faster, Smaller, Stronger; Practical Approaches for In Situ
X-ray Crystallography: from High-throughput Screening to Serial Data Collection; Delivery of GPCR Crystals for Serial Femtosecond Crystallography; The Mesh&Collect Pipeline for the Collection of Multi-crystal Data Sets in Macromolecular Crystallography; Radiation Damage in Macromolecular Crystallography; Data Quality Analysis; Structure Determination at Low-resolution, Anisotropic Data and Crystal Twinning; Structure Determination and Refinement of Large Macromolecular Assemblies at Low Resolution; Crystallography with X-ray Free Electron Lasers
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