Thomas Kohler & Dirk Laner 
Quantitation of mRNA by Polymerase Chain Reaction [PDF ebook] 
Nonradioactive PCR Methods

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In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; m RNA purification; c DNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

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Language English ● Format PDF ● ISBN 9783642797125 ● Publisher Springer Berlin Heidelberg ● Published 2012 ● Downloadable 3 times ● Currency EUR ● ID 6333561 ● Copy protection Adobe DRM
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